Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrase-On-Demand: bioprospecting integrases for targeted genomic insertion of genetic cargo.

Nucleic acids research·2026
Same author

Cryptic phosphorylation sites provide new structural and functional insights into the human phosphoproteome.

Trends in biochemical sciences·2026
Same author

Engineered orthogonal translation systems from metagenomic libraries expand the genetic code.

bioRxiv : the preprint server for biology·2025
Same author

Synthetic Genetic Elements Enable Rapid Characterization of Inorganic Carbon Uptake Systems in <i>Cupriavidus necator</i> H16.

ACS synthetic biology·2025
Same author

Engineering a genomically recoded organism with one stop codon.

Nature·2025
Same author

Structural basis for human NKCC1 inhibition by loop diuretic drugs.

The EMBO journal·2025
Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026
Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026
Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026
Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026
Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026
Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jun 13, 2025

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.4K

Genomically recoded Escherichia coli with optimized functional phenotypes.

Colin Hemez1,2,3, Kyle Mohler1,4, Felix Radford1,2

  • 1Systems Biology Institute, Yale University, West Haven, CT 06516.

Biorxiv : the Preprint Server for Biology
|September 11, 2024
PubMed
Summary
This summary is machine-generated.

Genetically engineered microbes ( genomically recoded organisms) can be improved by fixing underlying mutations. This study optimized *Escherichia coli* C321.∆A, enhancing its growth and function for biotechnology.

More Related Videos

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.5K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.1K

Related Experiment Videos

Last Updated: Jun 13, 2025

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.4K
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.5K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.1K

Area of Science:

  • Synthetic Biology
  • Microbial Engineering
  • Genomics

Background:

  • Genomically recoded organisms offer biotechnological potential but often suffer from reduced fitness.
  • Understanding the genetic basis of these fitness defects is crucial for strain improvement.

Purpose of the Study:

  • To identify and address the causes of fitness impairment in the genomically recoded *Escherichia coli* C321.∆A.
  • To engineer improved strains with enhanced growth kinetics and functional capabilities.

Main Methods:

  • Targeted metabolic screening
  • Genetic analysis
  • Proteomics
  • Phenotypic characterization

Main Results:

  • Identified isoleucine biosynthesis and release factor activity defects as key fitness limitations.
  • Engineered strains showed significant reductions in doubling time (17% in rich, 42% in minimal media).
  • Optimized strains exhibited enhanced non-standard amino acid incorporation and restored metabolic pathway regulation.

Conclusions:

  • Genome recoding can exacerbate pre-existing suboptimal traits in microbial hosts.
  • Correcting specific mutations can rapidly and precisely optimize the phenotype of engineered microbes.
  • This strategy is applicable for improving genomically recoded organisms and other engineered microbes for biotechnological applications.