Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

2.2K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
2.2K
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

6.3K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
6.3K
The Resting Membrane Potential01:21

The Resting Membrane Potential

131.5K
Overview
131.5K
Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

3.4K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.4K
Resting Membrane Potential01:24

Resting Membrane Potential

18.3K
The relative difference in electrical charge, or voltage, between the inside and the outside of a cell membrane, is called the membrane potential. It is generated by differences in permeability of the membrane to various ions and the concentrations of these ions across the membrane.
The Inside of a Neuron is More Negative
The membrane potential of a cell can be measured by inserting a microelectrode into a cell and comparing the charge to a reference electrode in the extracellular fluid. The...
18.3K
Tight Junctions01:29

Tight Junctions

5.2K
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adapted Smart-seq3xpress Facilitates Selective Microglial Transcriptomic Profiling From Frozen Brain Tissue.

Cellular and molecular neurobiology·2026
Same author

Reply to: On the interpretation of astrocytic calcium signalling with graphene oxide electrodes.

Nature nanotechnology·2026
Same author

The molecular basis of force selectivity by PIEZO2.

Nature·2026
Same author

Applications of Spatial Transcriptomics in Ischemic Stroke Research.

The American journal of pathology·2026
Same author

TRPV4 regulates intraocular pressure through trabecular meshwork contractility and fibrosis.

Channels (Austin, Tex.)·2026
Same author

Mechano- and Glucocorticoid-Sensitive TREK-1 Channels Regulate Conventional Outflow and Intraocular Pressure.

Investigative ophthalmology & visual science·2025

Related Experiment Video

Updated: Jun 13, 2025

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
09:03

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

10.0K

Resting human trabecular meshwork cells experience tonic cation influx.

Oleg Yarishkin1, Monika Lakk1, Christopher N Rudzitis1

  • 1University of Utah.

Research Square
|September 11, 2024
PubMed
Summary

Trabecular meshwork (TM) cells maintain resting membrane potential via a novel cation leak current, crucial for regulating intraocular pressure (IOP). This current, unlike known channels, influences cellular signaling and ionic balance.

Keywords:
Ion channelTRPC1calcium signalingglaucomamembrane potentialtrabecular meshwork

More Related Videos

Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents
08:30

Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents

Published on: January 31, 2020

7.2K
Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

9.4K

Related Experiment Videos

Last Updated: Jun 13, 2025

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
09:03

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

10.0K
Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents
08:30

Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents

Published on: January 31, 2020

7.2K
Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

9.4K

Area of Science:

  • Ophthalmology
  • Cell Physiology
  • Ion Channel Biology

Background:

  • The trabecular meshwork (TM) regulates intraocular pressure (IOP) by sensing mechanical and biochemical cues.
  • Understanding the ion channels responsible for ionic homeostasis in resting TM cells is critical but largely unknown.
  • Electrophysiological studies suggest pressure-sensing ion channels mediate TM cell signaling.

Purpose of the Study:

  • To identify the ion channels responsible for the resting membrane potential and ionic homeostasis in TM cells.
  • To characterize the properties of the constitutive cationic conductance in TM cells.
  • To investigate the role of TRP channels in TM cell resting potential.

Main Methods:

  • Electrophysiology to measure TM cell resting potential and ionic currents.
  • Ion substitution experiments to identify charge carriers (e.g., Na+).
  • Pharmacological profiling using channel blockers and activators (e.g., TTX, verapamil, SEA-0440, Gd3+, Ruthenium Red, GsMTx4, Pyr3, Pico1,4,5, SKF96365).
  • Transcriptional analysis to detect TRP gene expression.

Main Results:

  • A powerful cationic conductance, dependent on Na+, underlies the TM resting potential.
  • This conductance is distinct from voltage-gated Na+/Ca2+ channels, ENaC, and Na+/H+ exchange.
  • TRP-like channels, particularly TRPC1, are implicated, though classical TRPC blockers had unexpected effects.
  • A novel constitutive monovalent cation leak current, not fully characterized by known TRP channels, was identified.

Conclusions:

  • TM cells possess a unique cation leak current essential for maintaining resting membrane potential and Na+ homeostasis.
  • This novel current, potentially TRP-related but molecularly unidentified, influences TM cell signaling and IOP regulation.
  • Further research is needed to identify the molecular identity of this critical ion channel.