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Updated: Jul 4, 2026
07:05
Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer
Published on: September 22, 2015
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Biofabrication & cryopreservation of tissue engineered constructs for on-demand applications
Harshavardhan Budharaju1, Dhakshinamoorthy Sundaramurthi1, Swaminathan Sethuraman1
1Tissue Engineering & Additive Manufacturing (TEAM) Lab, Centre for Nanotechnology & Advanced Biomaterials, ABCDE Innovation Centre, School of Chemical & Biotechnology, SASTRA Deemed University, Thanjavur, Tamil Nadu, India.
Biofabrication
|September 11, 2024
Summary
Cryopreservation techniques are emerging to preserve 3D bioprinted tissues for on-demand applications. This review explores fabrication methods and cryopreservation factors for viable tissue engineered products.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Tissue Engineering
Background:
- Conventional scaffold-based methods create tissue engineered constructs for repair.
- 3D bioprinting offers precision in fabricating artificial tissues for biomedical applications.
- Current limitations exist in preserving bioprinted constructs for on-demand use due to cell viability challenges.
Purpose of the Study:
- To review fabrication techniques for tissue engineered products.
- To discuss cryopreservation strategies for bioprinted tissues.
- To highlight the potential of cryopreservable bioprinted tissues in healthcare.
Main Methods:
- Review of fabrication techniques: electrospinning, hydrogels, 3D bioprinting.
- Analysis of factors influencing bioprinted tissue cryopreservation: cryoprotectants, polymers, crosslinking, viscoelastic properties, storage.
- Elaboration on healthcare applications of cryopreservable bioprinted tissues.
Main Results:
- Various fabrication methods like electrospinning and 3D bioprinting are suitable for tissue engineering.
- Cryopreservation is crucial for maintaining cell viability and functionality of bioprinted tissues.
- Key factors influencing successful cryopreservation include cryoprotectant choice, polymer properties, and storage conditions.
Conclusions:
- Cryopreservation of 3D bioprinted tissues enables on-demand applications in regenerative medicine.
- Further research is needed to optimize fabrication and cryopreservation protocols.
- Cryopreservable bioprinted tissues hold significant promise for future healthcare solutions.
Keywords:
bioprintingcryopreservationelectrospinninghydrogelsready-to-usetissue engineered constructsMore Related Videos
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