VvbHLH036, a basic helix-loop-helix transcription factor regulates the cold tolerance of grapevine
Yujun Hou1,2, Darren Chern Jan Wong3,4, Xiaoming Sun1
1CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture/Center of Economic Botany, Core Botanical Gardens/Sino-Africa Joint Research Center, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
Plant Physiology
|September 11, 2024
Summary
Grapevine cold tolerance is enhanced by VvbHLH036, a transcription factor regulating threonine biosynthesis via VvThrC1. This pathway improves cold stress response and amino acid accumulation in grapes.
Area of Science:
- Plant Science
- Molecular Biology
- Agricultural Science
Background:
- Cold stress significantly limits grapevine (Vitis vinifera) growth and productivity.
- Understanding molecular mechanisms of cold tolerance is crucial for crop improvement.
Purpose of the Study:
- To identify and characterize grapevine genes involved in cold stress response.
- To elucidate the regulatory role of VvbHLH036 and its downstream targets in cold tolerance.
Main Methods:
- Gene identification and characterization (VvbHLH036).
- Gene overexpression and CRISPR/Cas9-mediated knockout (KO) in grapevine.
- Transcriptome analysis and promoter binding assays.
- Measurement of threonine content and reactive oxygen species (ROS).
Main Results:
- VvbHLH036 positively regulates cold tolerance in grapevine roots.
- VvbHLH036 directly activates the expression of threonine synthase (VvThrC1).
- Cold stress induces VvThrC1 and threonine accumulation, regulated by VvbHLH036; exogenous threonine enhances cold tolerance.
Conclusions:
- VvbHLH036 and VvThrC1 are key regulators of threonine biosynthesis and cold stress response in grapes.
- This pathway enhances cold tolerance by modulating amino acid levels and reducing oxidative damage.
- Findings offer potential targets for developing cold-hardy grapevine varieties.
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