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Updated: May 6, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
High frequency of silent mutations in gyrA gene of Mycobacterium tuberculosis in Indian isolates
Anamika Gupta1, Sudhir K Pal1, Vijay Nema1
1Division of Molecular Biology, ICMR-National Institute of Translational Virology and AIDS Research (formerly: National AIDS Research Institute), Pune, Maharashtra, India.
Abstract:
Reporting any uncommon or untapped changes in bacterial genetics or physiology would be of great importance to support the drug development process. We studied 120 Mycobacterium tuberculosis clinical isolates with different geographical origin within India and their resistance profile and found a significant number of isolates (109) harboring the polymorphism at nucleotide positions 61 and 284 of the gyrA gene. Bioinformatics analysis of these changes for drug binding suggested no significant change in the binding of the drug but have lower binding energies as compared with the wild-type proteins. Although functionally silent for the gyrA gene, these changes are indicating a silent geographical and evolutionary change that needs to be further studied for drug discovery and bacterial fitness.
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