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Research into overcoming drug resistance in lung cancer treatment using CRISPR-Cas9 technology: a narrative review

Bin Liu1, Ziyu Wang1, Meng Gu1

  • 1Cancer Research Center, Beijing Chest Hospital, Capital Medical University/Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.

Translational Lung Cancer Research
|September 12, 2024
PubMed
Abstract

Insights

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 gene editing offers a precise method to combat lung cancer drug resistance by targeting key genes. This technology shows promise for developing personalized therapies and improving patient outcomes.

Area of Science:

  • Oncology
  • Genetics
  • Biotechnology

Background:

  • Lung cancer is a leading cause of cancer mortality worldwide.
  • Drug resistance is a major obstacle in effective lung cancer treatment.
  • CRISPR-Cas9 gene editing presents a novel approach to address drug resistance.

Purpose of the Study:

  • To review the application of CRISPR-Cas9 technology in understanding and overcoming drug resistance in lung cancer.
  • To summarize research progress on using CRISPR-Cas9 for lung cancer treatment strategies.

Main Methods:

  • A comprehensive literature search was performed on Web of Science and PubMed.
  • Keywords included CRISPR-Cas9, lung cancer, drug resistance, gene editing, and gene therapy.
  • Studies published between 2002 and September 2023 focusing on CRISPR-Cas9 and lung cancer drug resistance were analyzed.

Main Results:

  • CRISPR-Cas9 enables precise gene editing for targeting resistance mechanisms in lung cancer.
  • The technology has been applied to key genes like EGFR, KRAS, TP53, and BCL2.
  • CRISPR-Cas9 has demonstrated potential in inhibiting tumor growth, repairing mutations, and enhancing chemotherapy sensitivity.

Conclusions:

  • CRISPR-Cas9 technology holds significant promise for advancing lung cancer treatment by addressing drug resistance.
  • Precise gene editing offers a powerful tool for developing personalized and more effective therapies.
  • Ongoing research aims to overcome challenges in delivery, safety, and ethics to enhance CRISPR-Cas9's role in improving patient outcomes.
Keywords:
Clustered regularly interspaced short palindromic repeats and clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas9)drug resistancegene editinggene therapylung cancer

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