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Amorphous PtO-engineered Pt@WO3 nanozymes with efficient NAD+ generation for an electrochemical cascade biosensor
Xinting Liu1, Wanyi Zhang1, Minghui Yang1,2,3
1Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. yangminghui@csu.edu.cn.
Chemical Communications (Cambridge, England)
|September 12, 2024
Summary
Platinum oxide structures enhance NADH oxidase-like activity in Pt@WO3 nanosheets, improving NAD+ regeneration for biosensing. This advancement enables sensitive detection of beta-hydroxybutyrate (HB) using electrochemical biosensors.
Area of Science:
- Materials Science
- Electrochemistry
- Biochemistry
Background:
- Nicotinamide adenine dinucleotide (NAD+) is crucial for metabolic pathways.
- Developing efficient NAD+ regeneration mimics for biosensing is challenging.
- NADH oxidase-like (NOX) mimics are promising for NAD+-dependent biosensors.
Purpose of the Study:
- To investigate the role of PtOx structures in Pt@WO3 nanosheets for NOX-like activity.
- To enhance NADH oxidation for NAD+ regeneration in electrochemical biosensing.
- To develop a sensitive electrochemical biosensor for beta-hydroxybutyrate (HB) detection.
Main Methods:
- Synthesis of Pt@WO3 nanosheets with incorporated PtOx structures.
- Characterization of PtOx formation via lattice oxygen translocation.
- Evaluation of nanozyme activity for NADH oxidation.
- Fabrication and testing of an electrochemical cascade biosensor for HB detection.
Main Results:
- PtOx formation via lattice oxygen translocation from WO3 to Pt NPs was observed.
- PtOx incorporation modulated Pt valence, creating active sites for NADH oxidation.
- Pt/PtOx@WO3- nanozymes exhibited enhanced NOX-like activity compared to Pt@WO3.
- An electrochemical biosensor using 650-Pt/PtOx@WO3- achieved a 25 μM detection limit for HB.
Conclusions:
- PtOx structures are key to activating NOX-like functionality in Pt-based nanozymes.
- This study provides insights into PtOx activation mechanisms for NOX mimics.
- The findings support the development of advanced NAD+/NADH-dependent electrochemical biosensors.
