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Updated: Jun 13, 2025
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
Amorphous PtO-engineered Pt@WO3 nanozymes with efficient NAD+ generation for an electrochemical cascade biosensor
Xinting Liu1, Wanyi Zhang1, Minghui Yang1,2,3
1Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. yangminghui@csu.edu.cn.
Abstract:
Bioactive NAD+ mediated multiple biocatalytic pathways in metabolic networks. Refining the structure of NADH oxidase-like (NOX) mimics to efficiently replenish NAD+ has been promising but challenging in NAD+-dependent dehydrogenase electrochemical cascade biosensing. Herein, we discovered that PtO structures, formed via lattice oxygen translocation from WO3 to Pt NPs at the interface, potentially activate and modulate the NOX-like functionality in Pt@WO3 nanosheets. Incorporating PtO leads to a more positive valence of Pt species within Pt/PtO@WO3-, where the PtO2 species serve as preeminent reaction sites for NADH coordination, activation, and dehydrogenation. Consequently, such nanozymes display enhanced NOX-like activity towards NADH oxidation in comparison to Pt@WO3. Ultimately, the 650-Pt/PtO@WO3- nanozyme is employed in an electrochemical cascade biosensor for β-hydroxybutyrate (HB) detection, achieving a calculated detection limit of 25 μM. This study offers insights into PtO activation in Pt-based NOX mimics and supports the future development of NAD+/NADH-dependent electrochemical biosensors.
