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Dye-Based Fluorescent Organic Nanoparticles, New Promising Tools for Optogenetics.

Jérémy Lesas¹, Thomas C M Bienvenu², Eleonore Kurek³

¹Neurocentre Magendie, INSERM U1215, Université de Bordeaux, Bordeaux, 33000, France.

Advanced Healthcare Materials

|September 12, 2024

Summary

This summary is machine-generated.

Dye-based fluorescent organic nanoparticles offer a bright, biocompatible platform for in vivo optogenetics. These nanoparticles successfully reduced fear responses in mice by manipulating neurons, showing potential for neurological disorder treatments.

Keywords:

closed‐loop fluorescence neurostimulation optogenetic organic nanoparticles two‐photon absorption

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Area of Science:

Nanotechnology
Biomedical Engineering
Neuroscience

Background:

Dye-based fluorescent organic nanoparticles are synthesized via nanoprecipitation of pure dyes.
Their photophysical and colloidal properties depend on dye aggregation.
Their brightness and near-infrared emission make them suitable for in vivo applications.

Purpose of the Study:

To investigate the use of dye-based fluorescent organic nanoparticles for in vivo optogenetics.
To demonstrate their photophysical properties, biocompatibility, and ability to activate Chrimson opsin.
To present an example of their application in modulating fear responses.

Main Methods:

Nanoparticle synthesis through nanoprecipitation.
Characterization of photophysical properties and biocompatibility.
In vivo optogenetic stimulation using fluorescence reabsorption to activate Chrimson opsin.
Closed-loop stimulation for fear response modulation in mice.

Main Results:

Demonstrated photophysical properties and biocompatibility of the nanoparticles.
Confirmed activation of Chrimson opsin in vivo via fluorescence reabsorption.
Successfully reduced fear responses in mice by selectively manipulating fear-related neurons.
Showcased the nanoparticles' potential in a closed-loop optogenetic system.

Conclusions:

Dye-based fluorescent organic nanoparticles are a versatile platform for in vivo optogenetics.
These nanoparticles can effectively activate opsins and modulate neural circuits.
This approach holds significant promise for translational neuroscience and treating neurological disorders.