Assessment of beverage quality for ethyl caproate and procyanidin B2 utilizing binary liposomes

Procyanidins are bioactive compounds present in fruits like apples. These compounds can be extracted and studied for their functional properties. Within the class of procyanidins, procyanidin B2 (PB2) serves as a standard reference. Sake, a traditional Japanese alcoholic beverage, contains ethyl caproate (EC), which adds economic value to the drink. Analyzing PB2 in apple juice and EC in sake, particularly in simple prepared beverages without the use of organic solvents or in their original state, requires the application of binary liposomes composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). In this study, I investigated the size of liposomes and the ratio of solid ordered (So) to liquid disordered (Ld) liposomes. The results indicated that PB2 increased the size of liposomes, while EC decreased it. Additionally, EC influenced the ratio of So/Ld liposomes. These findings provide a foundational understanding for further research on the biophysical and physiological properties of EC and PB2.