Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 13, 2025

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

Towards quantifying plasmid similarity.

William Matlock¹, Liam P Shaw², Samuel K Sheppard²

¹Nuffield Department of Medicine, University of Oxford, Oxford, UK.

Microbial Genomics

|September 12, 2024

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A standardized core genome multilocus sequence typing and life identification number barcoding framework for global <i>Pasteurella multocida</i> surveillance and outbreak investigation.

Microbial genomics·2026

Same author

Protocols for genomic epidemiology and source attribution of enteric bacteria causing diarrhoeal disease across Africa.

BMJ open·2026

Same author

Whole Genome Characterization of Klebsiella Strains in European Hedgehogs and Human Nosocomial Settings Identified Shared Sequence Types, Antimicrobial Resistance Genes and Plasmids.

Zoonoses and public health·2026

Same author

DNA modifications of Durham Collection phages and promiscuity of GmrSD-family Type IV restriction enzyme BrxU.

Applied and environmental microbiology·2026

Same author

GeneScanner: profiling genetic variation across bacterial populations.

Microbial genomics·2026

Same author

Pathoadaptive evolution and clonal dissemination of community- associated methicillin-resistant Staphylococcus aureus in Egypt.

BMC infectious diseases·2026

Same journal

New isolates from the 1970s to early 2000s provide insights into the evolution of <i>Acinetobacter baumannii</i> international clone 2 and its resistome.

Microbial genomics·2026

Same journal

Comprehensive identification of sequence types belonging to <i>Acinetobacter baumannii</i> clonal complexes.

Microbial genomics·2026

Same journal

Genomic outbreak investigation of biosafety-level-3 pathogens using nanopore sequencing.

Microbial genomics·2026

Same journal

Genome mining and phylogenomics reveal diverse biosynthetic gene clusters in desert Actinobacteria.

Microbial genomics·2026

Same journal

Genomic insights into the resistome, mobilome and functional adaptation of <i>Achromobacter xylosoxidans</i> across clinical and environmental contexts.

Microbial genomics·2026

Same journal

One Health genomic surveillance identified high-risk carbapenem-resistant ST821 clones of <i>Acinetobacter baumannii</i> in Nigerian clinical and community settings.

Microbial genomics·2026

See all related articles

Quantifying plasmid similarity is crucial for understanding how resistance genes spread in pathogens. This study addresses challenges in analyzing vast plasmid data to define similarity thresholds for related plasmids.

Area of Science:

Microbiology
Genomics
Bioinformatics

Background:

Plasmids are extrachromosomal DNA elements that facilitate the rapid spread of antibiotic resistance and virulence genes among clinical pathogens.
Plasmid diversity is structured, featuring conserved gene backbones and variable genetic cargo, alongside genomic plasticity and frequent rearrangements.

Purpose of the Study:

To address the challenge of quantifying plasmid similarity amidst a data deluge.
To explore theoretical and practical considerations for defining related plasmid groups.
To establish appropriate similarity thresholds for plasmid analysis.

Main Methods:

Analysis of large-scale plasmid sequence data.
Investigation of plasmid genome structure and variation.

Keywords:

antimicrobial resistance genomic epidemiology mobile genetic elements

More Related Videos

Use of In Vivo Assembly for High-efficiency Plasmid Construction

Use of In Vivo Assembly for High-efficiency Plasmid Construction

Published on: February 7, 2025

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

Published on: January 28, 2018

Related Experiment Videos

Last Updated: Jun 13, 2025

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

Use of In Vivo Assembly for High-efficiency Plasmid Construction

Use of In Vivo Assembly for High-efficiency Plasmid Construction

Published on: February 7, 2025

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

Published on: January 28, 2018

Development of theoretical frameworks for plasmid relatedness.

Main Results:

Plasmid diversity exhibits a structured pattern with conserved backbones and adaptable gene content.
Plasmid genomes demonstrate significant structural plasticity and undergo frequent rearrangements.
Challenges exist in analyzing extensive plasmid data to determine similarity.

Conclusions:

Defining plasmid similarity requires both sampling natural variation and theoretical considerations.
Establishing robust similarity thresholds is essential for rationalizing plasmid data.
Further research is needed to effectively analyze and categorize plasmids.