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Laccase surface-display for environmental tetracycline removal: From structure to function.

Wei Han1, Ying Zhao1, Qi Chen1

  • 1School of Resources and Environment, Northeast Agricultural University, HarBin, Heilongjiang Province, 150030, PR China.

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This study explores using surface-displayed bacterial laccase (CotA) for efficient tetracycline degradation. The engineered bacteria show enhanced environmental remediation capabilities, even improving soil health.

Keywords:
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Area of Science:

  • Environmental Science
  • Biotechnology
  • Bioremediation

Background:

  • Tetracycline pollution poses significant environmental challenges.
  • Developing efficient and eco-friendly treatment methods is crucial.

Purpose of the Study:

  • Investigate laccase surface-display technology for tetracycline removal.
  • Compare bacterial laccase (CotA) with fungal laccase (Lacc6) for efficiency.
  • Assess the potential of surface-displayed bacteria for environmental remediation.

Main Methods:

  • Utilized Bacillus subtilis spore coat protein (CotA) for laccase surface display.
  • Compared degradation efficiency of surface-displayed CotA with intracellular expression.
  • Evaluated the tolerance of engineered bacteria to environmental stressors.
  • Analyzed the impact of treatment on soil enzyme activity and nitrogen cycling genes.

Main Results:

  • CotA demonstrated higher laccase activity, stability, and tetracycline degradation efficiency than Lacc6.
  • Surface-displayed bacteria (pGSA-CotA) showed superior degradation rates compared to intracellular expression.
  • Engineered bacteria exhibited tolerance to high temperatures, pH, and heavy metals.
  • Treated soil showed alleviated tetracycline stress, increased soil enzyme activity, and enhanced nitrogen cycling genes.

Conclusions:

  • Laccase surface-display technology is a promising strategy for environmental remediation of tetracycline.
  • Bacterial laccase (CotA) is more effective than fungal laccase for this application.
  • Surface-displayed bacteria can mitigate the negative impacts of tetracycline pollution on soil ecosystems.