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Virus inactivation using an electrically conducting virus filter in biopharmaceutical manufacturing process
Hoeun Jin1, Hyunsik Kim2, Soryong Chae2
1Department of Biological Sciences and Bioengineering, Inha University, 100 Inha-ro, Michuhol-gu, Incheon 22212, Republic of Korea.
New Biotechnology
|September 12, 2024
Summary
Innovative electrically conducting virus filters made from carbon veils (CV) offer reusable viral inactivation, reducing biopharmaceutical manufacturing costs and environmental impact while enhancing safety by preventing virus breakthroughs.
Area of Science:
- Biotechnology
- Materials Science
- Virology
Background:
- Biopharmaceutical manufacturing faces viral contamination risks, necessitating effective viral clearance during downstream processing.
- Conventional virus-retentive filters provide robust viral removal but are single-use, incurring costs and environmental concerns.
- Virus breakthroughs can occur with current filtration methods, compromising biopharmaceutical safety.
Purpose of the Study:
- To develop and evaluate an innovative, reusable electrically conducting virus filter for enhanced biopharmaceutical safety.
- To investigate the efficacy of electrical conduction for virus inactivation and filter reuse.
- To reduce costs and environmental impact associated with disposable virus filters.
Main Methods:
- Fabrication of electrically conducting virus filters using free-standing carbon veils (CV).
- Application of direct current heating to CV-assisted virus filters for virus inactivation.
- Assessment of filter reusability and structural integrity after multiple uses.
- Analysis of virus inactivation mechanisms, including phage capsid damage and genome elimination.
Main Results:
- The electrically conducting virus filter successfully inactivated bacteriophages by damaging their capsid and eliminating their RNA genome.
- The developed filter demonstrated reusability up to five times without significant changes to its physical or chemical structure.
- Electrical conduction through the carbon veil effectively inactivated viruses captured within the filter.
- The reusable filter design showed potential to prevent undesired virus breakthroughs.
Conclusions:
- Electrically conducting virus filters offer a promising reusable alternative to disposable filters in biopharmaceutical manufacturing.
- This technology enhances biopharmaceutical safety through effective viral inactivation and prevention of breakthroughs.
- The reusability of these filters significantly reduces process costs and environmental burden.
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