Exploring extrahepatic metastasis of hepatocellular carcinoma based on methylation driver genes and establishing a prognostic model for hepatocellular carcinoma

ShiLing Huang ¹, Yang Yang ², BoShu Ji ³

⁰Department of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150086, China.

Gene +

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September 12, 2024

View abstract on PubMed

Summary

This study identifies key DNA methylation drivers, DHX58 and EIF5A2, linked to hepatocellular carcinoma (HCC) metastasis and prognosis. A validated risk model aids in predicting patient outcomes for this common cancer.

Area Of Science

Oncology
Epigenetics
Bioinformatics

Background

Hepatocellular carcinoma (HCC) is a prevalent cancer with high mortality, often diagnosed late and prone to metastasis.
DNA methylation plays a critical role in epigenetic regulation and the development of cancer.

Purpose Of The Study

To identify DNA methylation driver genes associated with extrahepatic metastasis in HCC.
To develop and validate a prognostic model for HCC based on methylation drivers.

Main Methods

Utilized TCGA and GSE116174 datasets for differential methylation and expression analyses.
Identified methylation driver genes using correlation analysis (coefficient≥|0.30|, P<0.05).
Constructed and validated a prognostic model using Cox regression, incorporating a risk score and clinical factors. Immunohistochemistry and CIBERSORT were used for validation and immunoinfiltration analysis.

Main Results

Identified eight methylation driver genes for HCC extrahepatic metastasis, with DHX58 and EIF5A2 significantly associated with prognosis.
A validated risk score and prognostic model were established.
M0 macrophage abundance correlated with HCC patient prognosis. DHX58 and EIF5A2 expression differences were observed between metastatic and non-metastatic HCC tissues.

Conclusions

DHX58 and EIF5A2 are potential methylation drivers and prognostic biomarkers for HCC extrahepatic metastasis.
The developed prognostic model and risk score can aid in predicting HCC patient outcomes.
Macrophage infiltration may influence HCC prognosis, warranting further investigation.

Keywords:

Biomarkers Extrahepatic metastasis Hepatocellular carcinoma Methylation driver genes Prognosis model