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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Jun 13, 2025

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

476

Advances in CRISPR-Cas systems for epigenetics.

Mahnoor Ilyas1, Qasim Shah2, Alvina Gul3

  • 1Shifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, Islamabad, Pakistan; Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan.

Progress in Molecular Biology and Translational Science
|September 12, 2024
PubMed
Summary
This summary is machine-generated.

The CRISPR-Cas9 system enables precise epigenetic modifications, offering new avenues for understanding and treating diseases linked to DNA methylation and histone changes. This technology holds promise for future clinical applications in gene editing.

Keywords:
CRISPR-Cas9CRISPRaCRISPRiEpigeneticsHistone modifications

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Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Epigenetic modifications, including DNA methylation and histone alterations, are crucial in human disease pathogenesis.
  • While DNA and chromatin modifications are understood, RNA nucleotide alterations are gaining attention.
  • CRISPR-based epigenetic editing tools offer precise control over these modifications.

Purpose of the Study:

  • To explore the role of the CRISPR-Cas9 system in epigenetic modifications.
  • To highlight the potential of CRISPR-Cas9 for therapeutic applications in human diseases.
  • To discuss the advancements in CRISPR-based epigenetic editing for clinical use.

Main Methods:

  • Utilizing catalytically inactive Cas9 (dCas9) coupled with epigenetic modifiers (histone code editors, DNA methyltransferases).
  • Employing CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) for targeted epigenetic editing.
  • Focusing on selective DNA modification for maintaining epigenetic memory.

Main Results:

  • CRISPR-Cas9 systems demonstrate high efficacy in editing the epigenome of eukaryotic cells.
  • CRISPR-based tools allow for selective DNA modifications, preserving epigenetic memory.
  • Advancements facilitate the manipulation of CRISPR-Cas9 for clinical studies.

Conclusions:

  • The CRISPR-Cas9 system provides a powerful platform for epigenetic modifications.
  • Precise epigenetic editing is crucial for understanding and potentially treating diseases like cancer.
  • Optimizing delivery platforms will enhance the clinical utility of CRISPR-Cas9 in gene editing therapies.