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NeuroART: Real-Time Analysis and Targeting of Neuronal Population Activity during Calcium Imaging for Informed
Zac Bowen1,2, Dulara De Zoysa1,3, Kelson Shilling-Scrivo4,5
1Institute for Physical Science and Technology, University of Maryland, College Park, Maryland 20742.
Eneuro
|September 12, 2024
Summary
NeuroART software enables real-time analysis of neuronal activity and network function. This allows for precise optogenetic stimulation to investigate brain information processing and potentially inject information into the brain.
Area of Science:
- Neuroscience
- Computational Neuroscience
- Systems Neuroscience
Background:
- Two-photon calcium imaging enables large-scale neuronal activity monitoring in vivo.
- Holographic optogenetics offers precise control over neuronal populations.
- Real-time analysis is crucial for targeted optogenetic stimulation mimicking natural brain activity.
Purpose of the Study:
- To develop a software platform for real-time neuronal activity analysis and integrated optogenetic stimulation.
- To enable the identification of optimal stimulation targets based on dynamic functional network analysis.
- To facilitate the investigation of information processing and manipulation in the brain.
Main Methods:
- Development of NeuroART (Neuronal Analysis in Real Time) software.
- Integration of real-time neuronal activity readout with correlation and synchrony analysis.
- Compatibility with various microscope platforms via data stream access.
- Holographic photostimulation control using a spatial light modulator (SLM).
- Auditory stimuli used to demonstrate real-time inference of neuronal contribution to sensory processing.
Main Results:
- NeuroART provides real-time analysis of neuronal activity and network dynamics.
- Demonstrated real-time inference of neuronal contribution to auditory information processing.
- Successful integration of real-time analysis with holographic photostimulation for targeted network modification.
- NeuroART is compatible with diverse microscope systems without requiring hardware modification.
Conclusions:
- NeuroART enables advanced real-time analysis of neuronal populations for neuroscience research.
- The software facilitates targeted holographic optogenetics for investigating brain function.
- This platform supports collaborative research by offering broad microscope compatibility.
- NeuroART opens new possibilities for understanding and manipulating neural circuits.
