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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Jun 13, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

CRISPR for companion diagnostics in low-resource settings.

Xu Qian¹, Qiang Xu¹, Christopher J Lyon^2,3

¹Department of Clinical Laboratory, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou 310022, China. Qian_michelle2014@163.com.

|September 13, 2024

Summary

This summary is machine-generated.

Clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostics offer a promising new tool for point-of-care tests (POCTs). These advanced diagnostics can help expand disease screening for tuberculosis, cancers, and emerging infections, especially in low-resource settings.

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Area of Science:

Biotechnology
Molecular Diagnostics
Public Health

Background:

Significant mortality is linked to tuberculosis, multiple cancers, and emerging infectious diseases.
Current diagnostic methods often lack the accessibility and speed required for widespread screening, particularly in low-resource settings.
There is a critical need for novel point-of-care tests (POCTs) to enhance disease detection capacity.

Purpose of the Study:

To review clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostic (CRISPR-Dx) assay techniques applicable to POCT development.
To explore the potential of CRISPR-Dx assays in addressing the need for new POCTs in disease diagnosis.
To discuss current and future applications, opportunities, and challenges for CRISPR-Dx based POCTs.

Main Methods:

Review of existing and potential CRISPR-Dx assay techniques.
Analysis of sample processing, target amplification, multiplex assay design, and signal readout strategies.
Examination of applications in disease diagnosis and suitability for low-resource settings.

Main Results:

CRISPR-Dx assays present a versatile alternative to traditional nucleic acid tests for diagnostics.
These assays demonstrate strong potential for development into effective POCTs.
Various CRISPR-Dx techniques can be adapted for sample processing, amplification, multiplexing, and signal detection in POCTs.

Conclusions:

CRISPR-Dx assays hold significant promise for the development of new POCTs.
Advancements in CRISPR-Dx technology can expand screening for high-mortality diseases in diverse settings.
Further development is required to ensure CRISPR-Dx POCTs meet the criteria for broad implementation in low-resource environments.