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Updated: May 1, 2026

Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D
Published on: October 26, 2013
Sensitive label free imaging of 3D cell models with minimal toxicity using confocal reflectance
Michael A Taylor1, Jung Un Ally Choi1, Shiva Muthuswamy1
1Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, Queensland 4072, Australia. a.kijas@uq.edu.au.
Abstract:
Confocal reflectance imaging typically suffers from high background and poor sensitivity. We demonstrate sensitive and low-background reflectance imaging of cells encapsulated in transparent 3D hydrogels. Nanoscale cell morphology is visualized with sensitivity similar to confocal fluorescence, with low laser power, minimal specimen preparation, and reduced toxicity.
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