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Nanozyme-Based Microfluidic Chip System for pH-Regulated Pretreatment and Sensitive Sensing.

Yifei Chen1, Yu Wu1, Weiqing Xu1

  • 1State Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.

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This study introduces a nanozyme-based microfluidic chip that uses pH changes to improve cysteine detection. The system enhances interference resistance, enabling more accurate analysis of cysteine (Cys).

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Nanozymes offer catalytic activity for sensitive detection but suffer from low interference resistance.
  • Precise detection of target substances is often hindered by interfering compounds.
  • Developing robust nanozyme-based systems is crucial for reliable analytical applications.

Purpose of the Study:

  • To develop a nanozyme-based microfluidic chip system for pH-regulated pretreatment and sensitive detection of cysteine (Cys).
  • To enhance the interference resistance of nanozymes for accurate target substance detection.
  • To utilize the dual enzyme-like activities of copper metal-organic frameworks (Cu MOF) for selective Cys sensing.

Main Methods:

  • Immobilization of copper metal-organic frameworks (Cu MOF) onto a microfluidic chip.
  • Utilizing pH-regulated oxidase-like and laccase-like activities of Cu MOF.
  • Implementing a pH-regulated pretreatment step to eliminate interference from dopamine (DA).

Main Results:

  • The Cu MOF exhibited distinct cysteine oxidase-like activity at pH 7.0 and laccase-like activity at pH 8.0.
  • The developed nanozyme system effectively eliminated interference from dopamine (DA).
  • The system demonstrated significantly improved accuracy in detecting Cys, with an R² value of 0.9914.

Conclusions:

  • The pH-regulated nanozyme microfluidic chip enhances interference resistance for accurate Cys detection.
  • This approach provides an efficient method for sample pretreatment using nanozymes.
  • The study broadens the application of nanozymes in analytical detection systems.