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Updated: Jun 13, 2025
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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
Published on: July 6, 2022
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Spatial omics advances for in situ RNA biology
Jingyi Ren1, Shuchen Luo1, Hailing Shi1
1Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Molecular Cell
|September 13, 2024
Summary
Spatial RNA biology is crucial for gene expression and cellular function. New high-resolution methods reveal RNA dynamics, processing, and interactions, advancing our understanding of biological processes and diseases.
Area of Science:
- Molecular Biology
- Genomics
- Cell Biology
Background:
- Spatial RNA regulation is fundamental to gene expression and cellular functions.
- Understanding RNA's location and dynamics offers insights into development, neurobiology, and disease.
- Diverse biological processes rely on precise spatial control of RNA molecules.
Purpose of the Study:
- To review methodologies for studying spatial RNA biology across subcellular, cellular, and tissue levels.
- To highlight recent advances in high-resolution RNA and multimodal biomolecule profiling.
- To explore how these advancements illuminate RNA spatiotemporal kinetics, processing, translation, and interactions.
Main Methods:
- Cell fractionation
- In situ and proximity labeling techniques
- Advanced imaging and spatially indexed next-generation sequencing (NGS)
- Spatially informed computational modeling
Main Results:
- Recent methods allow near-genome-scale profiling of RNA and other biomolecules at high spatial resolution.
- New discoveries have been made regarding RNA spatiotemporal kinetics, processing, and translation status.
- RNA-protein interactions within cells and tissues are being elucidated with unprecedented detail.
Conclusions:
- The evolving landscape of experimental and computational strategies reveals the complexity of spatial RNA biology.
- Subcellular resolution is now achievable, uncovering cellular and tissue heterogeneity.
- These advancements open new avenues for research in RNA biology and its role in health and disease.
Keywords:
RNA kineticsRNA localizationcell fractionationin situ sequencinglocalized RNA translationmultimodal integrationproximity labelingspatial RNA biologyspatial transcriptomicsspatial translatomicsMore Related Videos
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