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Related Experiment Video

Updated: Jun 13, 2025

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Exosomal transcript cargo and functional correlation with HNSCC patients' survival.

Joni Yadav1, Apoorva Chaudhary1, Tanya Tripathi1

  • 1Department of Zoology, Molecular Oncology Laboratory, University of Delhi (North Campus), Delhi, 110007, India.

BMC Cancer
|September 13, 2024
PubMed
Summary

Exosomes from HPV-positive and HPV-negative head and neck squamous cell carcinoma (HNSCC) carry distinct transcripts. These altered transcripts, including SGK1 and MAD1L1, correlate with HNSCC patient survival, suggesting potential as biomarkers.

Keywords:
Differentially exported transcripts (DETs)ExosomesHead and neck cancers (HNCs)Human papillomavirus (HPV)Illumina HiSeqThe Cancer Genome Atlas (TCGA)

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Area of Science:

  • Oncology
  • Molecular Biology
  • Exosome Research

Background:

  • Human Papillomavirus (HPV) status influences treatment outcomes in head and neck squamous cell carcinoma (HNSCC).
  • Understanding the molecular differences between HPV-positive and HPV-negative HNSCC is crucial for personalized medicine.

Purpose of the Study:

  • To characterize the distinct exosomal transcriptomic profiles of HPV-positive and HPV-negative HNSCC.
  • To investigate the association between these differentially exported transcripts (DETs) and HNSCC patient survival.

Main Methods:

  • Exosomes were isolated from HPV-positive (93VU147T) and HPV-negative (OCT-1) HNSCC cell lines.
  • Transcriptome analysis was performed using Illumina HiSeq X.
  • Publicly available data from The Cancer Genome Atlas (TCGA) was utilized to assess clinical relevance.

Main Results:

  • 3785 DETs were identified between HPV-positive and HPV-negative exosomes.
  • Pathways related to protein machinery, cellular redox, and neurological disorders were over-represented in HPV-positive exosomes.
  • High expression of SGK1 and MAD1L1 in exosomes correlated with poor HNSCC patient survival, as did FADS3, SGK3, and TESK2 in HPV-negative exosomes.

Conclusions:

  • Exosomes from HPV-positive and HPV-negative HNSCC cells harbor distinct transcriptomic cargo.
  • Altered transcripts show clinical relevance and correlate with patient survival, indicating potential as HNSCC biomarkers.
  • These findings highlight potential therapeutic targets for HNSCC treatment.