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Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
Published on: June 25, 2015
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Delaying production with prokaryotic inducible expression systems
Jasmine De Baets1, Brecht De Paepe1, Marjan De Mey2
1Centre for Synthetic Biology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium.
Microbial Cell Factories
|September 13, 2024
Summary
Two-stage fermentation optimizes molecule production by delaying it until sufficient cell mass is achieved. Autoinducible systems, like quorum sensing, offer a promising alternative to external triggers for improved bacterial growth and production efficiency.
Area of Science:
- Biotechnology
- Synthetic Biology
- Metabolic Engineering
Background:
- Bacterial engineering for molecule production can impair growth due to metabolic burden or toxicity.
- Two-stage fermentation mitigates these issues by delaying production until adequate cell mass is reached.
Purpose of the Study:
- To review external and autoinduction methods for two-stage fermentation.
- To discuss optimization strategies for induction processes.
- To explore future challenges and prospects of inducible expression systems.
Main Methods:
- Discussion of external induction triggers (chemical molecules, fermentation parameters).
- Exploration of autoinducible systems, including quorum sensing.
- Overview of tuning methods for induction process optimization.
Main Results:
- Various methods exist for two-stage fermentation, each with pros and cons.
- Chemically inducible systems are established, but autoinducible systems are gaining interest.
- Autoinducible systems, like quorum sensing, avoid external interference.
Conclusions:
- Two-stage fermentation offers flexibility in microbial production.
- Autoinducible systems present advantages over traditional methods.
- Quorum sensing and similar systems may shape future inducible expression strategies.
