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Total Cholesterol Determination Accuracy in Dried Blood Spots
Elena Bonet Estruch1, María J López-Lara2, Eva N Gutiérrez-Cortizo3
1Clinical Chemistry and Laboratory Medicine Department, Infanta Elena Hospital, 21007 Huelva, Spain.
Diagnostics (Basel, Switzerland)
|September 14, 2024
Summary
Total cholesterol analysis in dried blood spots offers a precise and reproducible method for screening familial hypercholesterolemia. This approach can be integrated into neonatal screening programs for early detection.
Area of Science:
- Clinical Chemistry
- Biochemistry
- Medical Diagnostics
Background:
- Dried blood spots (DBS) offer a convenient matrix for biochemical analysis.
- Familial hypercholesterolemia (FH) is a genetic disorder necessitating early detection.
- DBS analysis for total cholesterol can serve as a screening tool for FH.
Purpose of the Study:
- To evaluate the diagnostic accuracy of total cholesterol measurement in DBS using a manual enzymatic method.
- To compare DBS cholesterol levels with serum cholesterol levels determined by an automated reference method.
Main Methods:
- Collected 394 paired serum and DBS samples.
- Measured cholesterol using automated method for serum and manual enzymatic method for DBS.
- Assessed method agreement using Passing-Bablok regression and Bland-Altman analysis.
- Validated correlation formulas internally and externally.
Main Results:
- Within- and between-day coefficients of variation were below 10.14% and 14.09%, respectively.
- Passing-Bablok analysis showed a precision of 0.803 and accuracy of 0.96.
- Internal validation yielded a precision of 0.716.
- Positive and negative predicted values were 0.77 and 0.92, respectively, with the developed formula.
Conclusions:
- Total cholesterol analysis in DBS is precise and reproducible.
- The manual enzymatic method for DBS cholesterol is a reliable diagnostic tool.
- This method supports the integration of DBS into neonatal screening for FH.
