Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Five key challenges for the plasma membrane insertion of nicotinic acetylcholine receptors in cellular model systems.

Molecular and cellular biochemistry·2025

Same author

Phosphonylated tyrosine and cysteine disulfide adducts both generated from immunoglobulin G and human serum albumin indicate exposure to the nerve agent VX in vitro.

Analytical and bioanalytical chemistry·2025

Same author

Characterization of cell membrane fragments containing muscle type nAChR from Tetronarce californica after preparation using high pressure homogenization.

Toxicology letters·2025

Same author

Sulfonatocalix[4]arene-Based Scavengers for V-Type Nerve Agents with Enhanced Detoxification Activity.

Chemistry (Weinheim an der Bergstrasse, Germany)·2024

Same author

Development of a scintillation proximity assay for [<sup>3</sup>H]epibatidine binding sites of Tetronarce californica muscle-type nicotinic acetylcholine receptor.

Toxicology letters·2024

Same author

Concentration-dependent effects of the nerve agents cyclosarin and VX on cytochrome P450 in a HepaRG cell-based liver model.

Journal of applied toxicology : JAT·2024

Search research articles

Related Experiment Video

Updated: Jun 13, 2025

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Cell-Sonar, a Novel Method for Intracellular Tracking of Secretory Pathways.

Sabrina Brockmöller¹, Thomas Seeger¹, Franz Worek¹

¹Bundeswehr Institute of Pharmacology and Toxicology, 80937 Munich, Bavaria, Germany.

|September 14, 2024

Summary

This study introduces cell-sonar, a novel and affordable method for tracking protein expression changes within cells. Cell-sonar simplifies intracellular tracking for researchers studying cellular pathways and protein interactions.

Keywords:

cellular tracking nicotinic acetylcholine receptor protein homeostasis

More Related Videos

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Published on: June 20, 2018

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

Related Experiment Videos

Last Updated: Jun 13, 2025

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Published on: June 20, 2018

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Intracellular tracking is crucial for trafficking research but often relies on complex, expensive, and technically demanding methods.
Existing techniques typically require transgenic modifications and specialized equipment, limiting accessibility for many researchers.

Purpose of the Study:

To introduce a novel, simplified method for tracking intracellular protein expression changes.
To demonstrate the utility of this method, termed "cell-sonar", across various cellular compartments.

Main Methods:

Developed and applied "cell-sonar", a novel technique for tracking protein marker expression changes.
Utilized in-cell Western analyses on isogenic cell lines differing in target protein overexpression.
Investigated the trafficking of the human adult muscle-type nicotinic acetylcholine receptor.

Main Results:

Cell-sonar successfully tracked protein expression changes in the endoplasmic reticulum, Golgi apparatus, and endocytic pathways.
Demonstrated receptor maturation in the Golgi and storage in recycling endosomes.
Showed nicotine treatment exacerbates receptor degradation.

Conclusions:

Cell-sonar offers an affordable and easy-to-implement alternative for intracellular tracking.
The method is adaptable to diverse proteins and cellular pathways.
This technique can significantly aid researchers in studying protein trafficking and cellular dynamics.