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Toward DNA-Based Recording of Biological Processes
Hyeri Jang1, Sung Sun Yim1,2,3,4
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
International Journal of Molecular Sciences
|September 14, 2024
Summary
Engineered cellular memory systems use DNA storage for biological recording inside the body. This review covers technologies like CRISPR-Cas and discusses future improvements for applications in sensing and disease monitoring.
Area of Science:
- Biotechnology
- Molecular Biology
- Synthetic Biology
Background:
- DNA-based data storage offers inherent compatibility with living cells.
- Cellular recording systems enable data retrieval from inaccessible locations, including inside the body.
Purpose of the Study:
- To provide an overview of engineered cellular memory systems.
- To highlight design principles, advantages, and limitations of current technologies.
- To discuss strategies for enhancing recording accuracy, scalability, and durability.
Main Methods:
- Review of existing literature on cellular recording technologies.
- Examination of CRISPR-Cas systems, recombinases, retrons, and DNA methylation.
- Analysis of strategies for improving recording fidelity and longevity.
Main Results:
- Overview of diverse cellular recording technologies and their mechanisms.
- Identification of current limitations in accuracy, scalability, and durability.
- Discussion of potential advancements for future applications.
Conclusions:
- Engineered cellular memory systems are a promising modality for biological measurement.
- These systems offer novel insights into biological processes.
- Potential applications include environmental sensing and disease monitoring.
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