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Updated: Jun 13, 2025

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
Published on: June 2, 2020
Large Extracellular Vesicles Derived from Natural Killer Cells Affect the Functions of Monocytes
Dmitry Sokolov1,2, Alina Gorshkova1, Elizaveta Tyshchuk1,2
1Federal State Budgetary Scientific Institution "The Research Institute of Obstetrics, Gynecology and Reproductology Named after D.O. Ott", 199034 St. Petersburg, Russia.
Abstract:
Communication between natural killer cells (NK cells) and monocytes/macrophages may play an important role in immunomodulation and regulation of inflammatory processes. The aim of this research was to investigate the impact of NK cell-derived large extracellular vesicles on monocyte function because this field is understudied. We studied how NK-cell derived large extracellular vesicles impact on THP-1 cells characteristics after coculturing: phenotype, functions were observed with flow cytometry. In this study, we demonstrated the ability of large extracellular vesicles produced by NK cells to integrate into the membranes of THP-1 cells and influence the viability, phenotype, and functional characteristics of the cells. The results obtained demonstrate the ability of large extracellular vesicles to act as an additional component in the immunomodulatory activity of NK cells in relation to monocytes.
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