Aging-Related Metabolome Analysis of the Masseter Muscle in Senescence-Accelerated Mouse-Prone 8

Yoshiaki Kato ¹, Teruhide Hoshino ¹, Yudai Ogawa ²

⁰Department of Oral Pathobiological Science and Surgery, Tokyo Dental College, Tokyo 101-0061, Japan.

International Journal of Molecular Sciences +

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September 14, 2024

View abstract on PubMed

Summary

Aging alters masseter muscle metabolism, impacting frailty and sarcopenia. Key metabolic pathways like glycolysis and polyamine metabolism change, with increased spermidine and Val levels observed in aging mice.

Area Of Science

Gerontology
Metabolomics
Muscle Physiology

Background

Frailty and sarcopenia are critical in aging, affecting quality of life and transition to long-term care.
Oral function is increasingly recognized for its link to frailty and sarcopenia.
Understanding age-related muscle changes is crucial for developing interventions.

Purpose Of The Study

To investigate age-related changes in metabolites and metabolic pathways within the masseter muscle.
To identify specific metabolic shifts associated with aging in senescence-accelerated mouse-prone 8 (SAMP8) mice.

Main Methods

Capillary electrophoresis-mass spectrometry (CE-MS) metabolome analysis was performed on masseter muscle samples from 12-, 40-, and 55-week-old SAMP8 mice.
Reverse transcription polymerase chain reaction (RT-PCR) was used to confirm enzyme expression in aging-related metabolic pathways.

Main Results

Significant metabolic fluctuations were observed across the different age groups.
Key affected metabolic pathways included glycolysis, polyamine metabolism, and purine metabolism.
Increased levels of spermidine and Valine (Val) were identified as characteristic age-related changes in the masseter muscle.

Conclusions

Aging in SAMP8 mice significantly alters masseter muscle metabolism, involving glycolysis, polyamine, and purine pathways.
Elevated spermidine and Val levels in the masseter muscle are distinctive markers of aging compared to lower limb muscles.

Keywords:

aging masseter muscle metabolites metabolome analysis senescence-accelerated mouse