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The ATTO 565 Dye and Its Applications in Microscopy.
1Molecular Photonics Group, Van 't Hoff Institute for Molecular Sciences (HIMS), Universiteit van Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands.
Molecules (Basel, Switzerland)
|September 14, 2024
Summary
ATTO 565, a Rhodamine dye, offers excellent optical properties and low toxicity for advanced microscopy. This review explores its applications in biological imaging and future potential in biocompatibility and metal ion detection.
Area of Science:
- Biochemistry
- Optical Physics
- Microscopy
Background:
- ATTO 565 is a Rhodamine-type dye with high fluorescence quantum yield, stability, and low biotoxicity.
- Its unique optical properties make it suitable for advanced imaging techniques.
Purpose of the Study:
- To review the chemical and optical properties of ATTO 565.
- To discuss its functionality in various microscopy techniques.
- To explore future applications in biocompatibility and metal ion detection.
Main Methods:
- Review of existing literature on ATTO 565.
- Analysis of its performance in confocal microscopy, STED microscopy, single-molecule tracking (SMT), TPE-STED, and FCS.
- Discussion of its chemical and optical principles.
Main Results:
- ATTO 565 demonstrates significant utility in biological imaging and single-molecule localization.
- Its properties are well-suited for advanced microscopy applications.
- The dye shows promise for future applications.
Conclusions:
- ATTO 565 is a valuable tool in advanced microscopy and biological research.
- Further investigation into its potential applications is warranted.
- Its biocompatibility and metal ion detection capabilities are areas for future development.
Keywords:
3D single-molecule trackingfluorescence correlation spectroscopyfluorescent Rhodamine dyefluorescent labelinghigh-resolution microscopystimulated emission depletion microscopyMore Related Videos
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