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Mei5-Sae3 stabilizes Dmc1 nucleating clusters for efficient Dmc1 assembly on RPA-coated single-stranded DNA.

Chin-Dian Wei1, Hao-Yen Chang1,2, Chia-Hua Lu1

  • 1Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan.

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Summary
This summary is machine-generated.

The Mei5-Sae3 complex stabilizes DNA recombinase Dmc1 assembly on single-stranded DNA, facilitating replication protein A (RPA) displacement during meiosis.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Meiosis requires interhomolog recombination mediated by the Dmc1 recombinase.
  • The Mei5-Sae3 complex is crucial for loading Dmc1 onto replication protein A (RPA)-coated single-stranded DNA (ssDNA) in Saccharomyces cerevisiae.
  • The precise mechanism by which Mei5-Sae3 facilitates Dmc1 activity remains incompletely understood.

Purpose of the Study:

  • To elucidate the molecular mechanism by which the Mei5-Sae3 complex stimulates Dmc1 assembly on ssDNA.
  • To investigate the role of Mei5-Sae3 in Dmc1-mediated RPA displacement from ssDNA.
  • To understand the interplay between Dmc1, Mei5-Sae3, and RPA during meiotic recombination initiation.

Main Methods:

  • Single-molecule fluorescence resonance energy transfer (smFRET) spectroscopy.
  • Colocalization single-molecule spectroscopy.
  • Experiments utilizing green fluorescent protein-labeled RPA.

Main Results:

  • Mei5-Sae3 stabilizes Dmc1 nucleating clusters on naked ssDNA by reducing Dmc1 dissociation rates.
  • Mei5-Sae3 promotes Dmc1 assembly on RPA-coated ssDNA, leading to an intermediate state with both Dmc1 and RPA.
  • RPA displacement efficiency is dependent on Dmc1 concentration and positively correlated with the stability of Dmc1 clusters.

Conclusions:

  • Mei5-Sae3 stabilizes Dmc1 nucleating clusters, which is critical for efficient Dmc1 loading onto RPA-coated ssDNA.
  • Mei5-Sae3 modulates RPA dynamics on ssDNA, promoting RPA dissociation to facilitate Dmc1 filament formation.
  • This study proposes a molecular model for Mei5-Sae3's role in initiating meiotic recombination by stabilizing Dmc1 binding and promoting RPA removal.