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Trimodal Multiplexed Lateral Flow Test Strips Assisted with a Portable Microfluidic Centrifugation Device.

Man-Wen Wang1, Zong-Min Chen1, Yung-Chun Hsieh2

  • 1Department of Applied Chemistry, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan.

Analytical Chemistry
|September 14, 2024
PubMed
Summary

This study introduces a novel trimodal lateral flow assay (LFA) that enhances sensitivity and allows multiplexed detection. The innovative probe design improves diagnostic accuracy for biomarkers like CYFRA 21-1 and CA15-3.

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Medical Diagnostics

Background:

  • Lateral flow assays (LFAs) saw increased use during COVID-19 but often lack sensitivity and specificity compared to ELISA and PCR.
  • Previous attempts to improve LFAs have increased complexity, hindering large-scale point-of-care applications.
  • There is a need for more sensitive, specific, and multiplexed LFAs for diverse diagnostic applications.

Purpose of the Study:

  • To develop a novel trimodal probe for enhancing LFA sensitivity and selectivity.
  • To enable multiplexed detection of multiple analytes using a single LFA platform.
  • To demonstrate the feasibility of this platform for cancer biomarker detection.

Main Methods:

  • Developed a trimodal nanohybrid probe integrating fluorescence, color, and magnetic signals.
  • Utilized gold nanoparticles and polymer dots for enhanced optical detection.
  • Incorporated magnetic properties for accurate quantitative measurements.
  • Tested the platform using CYFRA 21-1 (lung cancer) and CA15-3 (breast cancer) biomarkers.

Main Results:

  • Achieved a remarkable detection limit of 0.26 ng/mL for CYFRA 21-1 and 2.8 U/mL for CA15-3.
  • Demonstrated the first trimodal LFA with multiplexing capability.
  • Validated the platform's accuracy and reliability using clinical serum samples, showing high consistency with electrochemiluminescence immunoassay.

Conclusions:

  • The developed trimodal LFA platform significantly enhances sensitivity and selectivity.
  • This universal platform enables multiplexed detection and has broad applicability for next-generation LFAs.
  • The approach offers a promising solution for advanced point-of-care diagnostics.