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Antithrombotic Revascularization Strategy of Bioengineered Liver Using a Biomimetic Polymer.

Hiroshi Horie1, Yu Oshima1,2, Ken Fukumitsu1,3

  • 1Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Tissue Engineering. Part A
|September 14, 2024
PubMed
Summary
This summary is machine-generated.

A new antithrombotic polymer reduces blood clot formation in bioengineered liver scaffolds. This innovation enhances scaffold safety and function, offering a promising solution for liver transplantation in patients with end-stage liver disease.

Keywords:
2-metacryloyloxyethyl phosphorylcholine polymerdecellularizationliver bioengineeringrevascularization

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Materials Science

Background:

  • End-stage liver disease necessitates innovative treatments like bioengineered livers.
  • Thrombogenicity (blood clot formation) remains a significant hurdle in developing functional bioengineered liver scaffolds.
  • Decellularized liver scaffolds offer a promising structural basis for bioengineered organs.

Purpose of the Study:

  • To develop and evaluate a novel antithrombotic polymer for revascularizing decellularized liver scaffolds.
  • To assess the thrombogenicity and biosafety of polymer-modified liver scaffolds.
  • To determine the impact of polymer treatment on scaffold function and recellularization.

Main Methods:

  • A biomimetic polymer, 2-methacryloyloxyethyl phosphorylcholine (MPC), was synthesized and applied to rat liver scaffolds.
  • The polymer's interaction with scaffold vessel walls was confirmed.
  • Ex vivo blood perfusion and heterotopic transplantation models were used to evaluate thrombogenicity and scaffold maintenance.
  • Metabolic function of recellularized hepatocytes within the scaffolds was assessed.

Main Results:

  • MPC polymer treatment significantly reduced platelet deposition in liver scaffolds compared to untreated or endothelial cell-treated scaffolds.
  • Polymer-treated scaffolds showed better liver volume maintenance and suppressed platelet deposition in transplantation models.
  • The scaffolds maintained the metabolic function of recellularized primary hepatocytes during perfusion culture.

Conclusions:

  • The MPC polymer effectively suppresses thrombus formation in bioengineered liver scaffolds during blood perfusion.
  • This antithrombotic modification improves scaffold biosafety and preserves the function of recellularized liver cells.
  • Revascularizing liver scaffolds with MPC polymer represents a promising strategy for advancing bioengineered liver transplantation.