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Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
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Extracellular vesicles in kidney disease - A veterinary perspective
Jack S Lawson1, Timothy L Williams2
1The Royal Veterinary College, Hawkshead Ln, Brookmans Park, Hatfield AL9 7TA, UK.
Veterinary Journal (London, England : 1997)
|September 14, 2024
Summary
Extracellular vesicles (EVs) are key to kidney health and injury, acting as messengers between cells. Urinary EVs in pets show promise as diagnostic biomarkers and therapeutic agents for kidney disease.
Area of Science:
- Renal physiology and pathophysiology
- Cellular biology and intercellular communication
- Veterinary medicine and comparative pathology
Background:
- Extracellular vesicles (EVs) are crucial mediators of intercellular communication in normal kidney function and disease.
- Urinary EVs (uEVs) contain cargo reflecting their cell of origin, offering insights into kidney health.
- Veterinary research utilizes animal models to explore EV roles in kidney injury and potential therapies.
Purpose of the Study:
- To review the biological functions of EVs in the kidney.
- To discuss techniques for isolating EVs, particularly urinary EVs (uEVs).
- To explore the potential of EVs as diagnostic biomarkers and therapeutic agents in kidney disease, with a focus on veterinary patients.
Main Methods:
- Review of existing literature on EV biology, isolation techniques, and applications in kidney disease.
- Analysis of studies investigating urinary EV proteome and transcriptome for biomarker potential.
- Examination of research on mesenchymal stromal cell-derived EVs for acute kidney injury treatment in veterinary models.
Main Results:
- EVs play significant roles in kidney physiology and injury, primarily through intercellular communication.
- Urinary EVs (uEVs) from cats and dogs can be isolated and analyzed for their proteome and transcriptome.
- Exogenous EVs have demonstrated efficacy in treating acute kidney injury in veterinary models, highlighting therapeutic potential.
Conclusions:
- Urinary EVs (uEVs) hold significant promise as non-invasive biomarkers for diagnosing kidney disease in veterinary patients.
- Bioengineered EVs offer a novel platform for targeted drug and nucleic acid delivery for kidney disease treatment.
- Further research into EVs in veterinary medicine can advance both diagnostic and therapeutic strategies for kidney disorders.
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