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Microwave energy fixation for electron microscopy.

G R Login, A M Dvorak

The American Journal of Pathology

|August 1, 1985

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Microwave energy (MW) rapidly fixes biological samples for electron microscopy in seconds. This new method preserves ultrastructure and proteins, enabling faster research into cellular processes.

Area of Science:

Electron microscopy
Cell biology
Biochemistry

Background:

Conventional fixation methods for electron microscopy are time-consuming.
Rapid fixation is crucial for studying dynamic cellular processes.

Purpose of the Study:

To develop and validate a rapid microwave (MW) energy-based fixation technique.
To assess the ultrastructural preservation and potential applications of MW fixation.

Main Methods:

Samples were fixed using microwave energy combined with chemical cross-linking agents.
Optimal conditions involved 9-second irradiation to 50°C for tissues up to 1 cu cm.
Ultrastructural preservation was compared to conventional aldehyde fixation.

Main Results:

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Microwave fixation achieved comparable ultrastructural preservation to 2-hour conventional fixation.
Stereologic analysis confirmed no significant organelle shrinkage or swelling.
The method is effective for preserving labile proteins like antigens and enzymes.

Conclusions:

Microwave-assisted fixation offers a rapid and effective alternative for electron microscopy.
This technique facilitates the study of rapid intracellular events and sensitive biomolecules.