Related Experiment Video
Updated: Jun 13, 2025

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
A novel fluorescent probe based on dicyanoisophorone derivatives for hypochlorite detection in living cells
Yibin Zhang1, Xiaoqian Rong1, Changjie Lin2
1College of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing, China.
Abstract:
This study presents a long-wavelength fluorescent probe CNC for the detection of ClO- in vitro and in vivo. Upon interaction with ClO-, this probe exhibited a significant increase in fluorescence, with a significant Stokes shift (169 nm), lower detection limit (1.38 μM), high sensitivity and selectivity. Moreover, the probe demonstrated excellent cell permeability and minimal cytotoxicity, allowing for successful imaging of both endogenous and exogenous ClO- in living cells.
More Related Videos
14:25Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells
Published on: May 13, 2022
07:42Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
Published on: October 13, 2015
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Photoluminescence: Applications