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Updated: Jun 13, 2025
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Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
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Ecofriendly approaches to efficiently enhance catalase performance.
M D Leonida1, I Kumar1, M R Elshaer1
1Department of Chemistry, Biochemistry and Physics, Fairleigh Dickinson University, Teaneck, NJ 07666, USA.
International Journal of Biological Macromolecules
|September 15, 2024
Summary
Two green methods enhance catalase (CAT) activity and stability by partially denaturing and refolding the enzyme with redox molecules. These modified enzymes show promise for biosensor applications.
Area of Science:
- Biochemistry
- Enzyme Engineering
- Green Chemistry
Background:
- Catalase (CAT) is crucial for antioxidant defense.
- Enhancing enzyme stability and activity is vital for industrial applications.
- Green chemistry approaches minimize environmental impact.
Purpose of the Study:
- To develop two novel, green methods for enhancing catalase activity and stability.
- To compare the efficacy of ionic liquid and high hydraulic pressure treatments.
- To investigate the potential of modified catalase in biosensor development.
Main Methods:
- Enzyme modification via transient ionic liquid (IL) exposure with redox molecules.
- Enzyme modification via high hydraulic pressure (HHP) treatment with redox molecules (reagentless).
- Dialysis for refolding and redox molecule entrapment, creating "wired" enzymes.
- Construction and testing of enzyme electrodes for hydrogen peroxide detection.
Main Results:
- Both IL and HHP methods successfully enhanced CAT activity and stability.
- Modified CAT retained catalytic effect and exhibited linear behavior in biosensors.
- The HHP method offers a reagentless approach to enzyme modification.
- Comparative analysis of parameters, residual activity, and redox molecule interactions was performed.
Conclusions:
- Simple, green processes can significantly enhance catalase performance.
- Modified catalase demonstrates potential for amperometric biosensors.
- Applications are foreseen in the food, pharmaceutical, and textile industries.
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