Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Colony assay for clinical application].

H Ichihashi, T Sako, S Akiyama

    Gan to Kagaku Ryoho. Cancer & Chemotherapy
    |August 1, 1985
    PubMed
    Summary

    Modified human tumor stem cell assays enable routine clinical drug sensitivity testing for advanced GI cancers. This method accurately predicts patient response to chemotherapy, aiding personalized treatment strategies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Accuracy of visually estimated left heart size by echocardiography in dogs with myxomatous mitral valve disease.

    Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology·2025
    Same author

    Non-genomic inhibitory effect of glucocorticoids on activated peripheral blood basophils through suppression of lipid raft formation.

    Clinical and experimental immunology·2012
    Same author

    Interface for coupling capillary electrophoresis to inductively coupled plasma and on-column concentration technique.

    Analytical chemistry·2011
    Same author

    Apnoea-hypopnoea index during rapid eye movement and non-rapid eye movement sleep in obstructive sleep apnoea.

    The Journal of international medical research·2008
    Same author

    [Evaluation of spinal cord ischemia in endovascular stent graft repair and surgical operation of descending thoracic or thoracoabdominal aortic aneurysms].

    Kyobu geka. The Japanese journal of thoracic surgery·2004
    Same author

    Metallic elements and metal poisoning among white-tailed sea eagles from the Baltic south coast.

    Marine pollution bulletin·2002

    Area of Science:

    • Oncology
    • Cancer Biology
    • Clinical Pharmacology

    Context:

    • The Salmon and Hamburger human tumor stem cell assay is a valuable tool for predicting anticancer drug efficacy.
    • Routine clinical application requires modifications for improved sample handling and drug administration.
    • Advanced gastrointestinal (GI) malignancies present challenges in predicting patient response to chemotherapy.

    Purpose:

    • To modify the human tumor stem cell assay for routine clinical use.
    • To evaluate the feasibility and accuracy of the modified assay in predicting drug sensitivity for advanced GI cancers.
    • To assess the impact of modified drug exposure methods on assay results, particularly for long-acting agents.

    Summary:

    • Modifications to the human tumor stem cell assay, including a 24-well multiplate system and pre-plating drug addition to agar, enhance feasibility for clinical studies.
    • The modified assay allows for accurate drug sensitivity testing, even with long-acting agents like 5-fluorouracil, and maintains colony-forming activity after freezer storage.
    • Testing on 32 patients with advanced GI malignancies revealed that 47% yielded sufficient colonies for drug sensitivity evaluation, with results correlating to clinical outcomes in some cases.

    Impact:

    • The modified assay facilitates routine clinical drug sensitivity testing, improving the selection of effective chemotherapies for patients with advanced GI cancers.
    • Successful prediction of drug sensitivity in two patients led to positive clinical responses, including ascites disappearance and significant regression of hepatic metastases.
    • The assay demonstrated both drug sensitivity and resistance in a significant proportion of patients, providing valuable prognostic information and guiding treatment decisions.

    Related Experiment Videos