Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Exosomes01:36

Overview of Exosomes

2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Diagnostic Potential Of Sdhb Mrna Contained In Serum Extracellular Vesicles Among Patients With Prostate Cancer

Diagnostic potential of SDHB mRNA contained in serum extracellular vesicles among patients with prostate cancer

Taku Kato1,2, Eiji Sugihara3, Yuko Hata3

  • 1Department of Joint Research Laboratory of Clinical Medicine, Fujita Health University School of Medicine, Toyoake, Japan.

The Prostate
|September 16, 2024

Related Experiment Videos

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
13:19

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

16.5K
Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
12:13

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

6.8K
Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections
07:55

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

Published on: November 8, 2024

399

View abstract on PubMed

Summary
This summary is machine-generated.

Serum extracellular vesicle (EV)-based biomarkers can help diagnose resistance to androgen receptor signaling inhibitors (ARSIs) in prostate cancer (PC). SDHB mRNA in EVs shows promise as a novel biomarker for early ARSI-resistance detection.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biomarker Discovery

Background:

  • Androgen receptor signaling inhibitors (ARSIs) are standard treatments for metastatic and castration-resistant prostate cancer (CRPC).
  • Acquired resistance to ARSIs remains a significant clinical challenge in prostate cancer management.
  • Novel biomarkers are needed for early diagnosis of ARSI resistance and identification of therapeutic targets.

Purpose of the Study:

  • To identify novel serum extracellular vesicle (EV)-based biomarkers for diagnosing ARSI resistance in CRPC.
  • To explore potential therapeutic targets for ARSI-resistant CRPC.

Main Methods:

  • Serum EVs were isolated from CRPC patients before and after ARSI treatment.
  • RNA sequencing was performed on EV RNA to identify expression changes.
Keywords:
CRPCSDHBandrogen signaling inhibitorsextracellular vesicles

Related Experiment Videos

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
13:19

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

16.5K
Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
12:13

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

6.8K
Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections
07:55

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

Published on: November 8, 2024

399
  • Quantitative reverse transcription PCR (RT-qPCR) and public datasets were used to validate findings in larger cohorts, including benign prostatic hyperplasia (BPH) and prostate cancer (PC) tissues.

    • Main Results:

    • Mitochondrial oxidative phosphorylation (OXPHOS)-related genes were upregulated in EVs from ARSI-resistant CRPC.
    • SDHB mRNA was significantly increased in EVs and tumor tissues after acquiring ARSI resistance.
    • EV-SDHB levels showed potential for early detection of ARSI resistance in patients.
    • SDHB mRNA expression in EVs correlated with tissue expression and other OXPHOS genes.

    Conclusions:

    • SDHB mRNA in serum EVs is a potential novel biomarker for diagnosing ARSI resistance in prostate cancer.
    • EV-derived SDHB mRNA levels correlate with tumor tissue expression.
    • EV-SDHB holds promise for the early diagnosis of ARSI resistance, guiding clinical treatment decisions.