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  1. Home
  3. The Inhibitory Effects Of The Novel Lactobacillus Cocktail On Colorectal Cancer Development Through Modulating Bmp Signaling Pathway: In Vitro And In Vivo Study.
  1. Home
  3. The Inhibitory Effects Of The Novel Lactobacillus Cocktail On Colorectal Cancer Development Through Modulating Bmp Signaling Pathway: In Vitro And In Vivo Study.

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The inhibitory effects of the novel Lactobacillus cocktail on colorectal cancer development through modulating BMP

Amin Sepehr1, Shadi Aghamohammad1, Roya Ghanavati2

  • 1Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.

Heliyon
|September 16, 2024

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
BMP signaling pathwayColorectal cancerLactobacillus cocktailProbiotics

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A five-strain Lactobacillus cocktail significantly reduced colorectal cancer cell proliferation and downregulated the bone morphogenetic proteins (BMP) signaling pathway. This probiotic intervention showed potential for colorectal cancer (CRC) prevention by inhibiting key pathway genes and beta-catenin.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Oncology

Background:

  • Colorectal cancer (CRC) remains a significant health concern, with aberrant signaling pathways contributing to its development.
  • The bone morphogenetic proteins (BMP) signaling pathway plays a complex role in cell differentiation and proliferation, and its dysregulation is implicated in various cancers.
  • Probiotics, particularly Lactobacillus species, are increasingly investigated for their potential health benefits, including anti-cancer properties.

Purpose of the Study:

  • To investigate the impact of a specific five-strain Lactobacillus cocktail on colorectal cancer (CRC) modulation.
  • To determine if the Lactobacillus cocktail affects the bone morphogenetic proteins (BMP) signaling pathway.
  • To evaluate the antiproliferative effects of the Lactobacillus cocktail in vitro and in vivo models of CRC.

Main Methods:

  • In vitro antiproliferative effects were assessed using the MTT assay on HT-29 cells.
  • An in vivo mouse model treated with azoxymethane (AOM)/dextran sulfate sodium (DSS) was used to mimic CRC conditions.
  • Gene expression analysis (qRT-PCR) and protein assessment (Western blotting) were employed to evaluate BMP signaling pathway activity and beta-catenin levels.

Main Results:

  • The Lactobacillus cocktail demonstrated significant, time-dependent inhibition of HT-29 cell proliferation in vitro.
  • Downregulation of the BMP signaling pathway and the hes1 gene was observed in treated cells and in the AOM/DSS-induced mouse model.
  • A marked decrease in beta-catenin protein expression was consistently found in both in vitro and in vivo experiments.

Conclusions:

  • The five-strain Lactobacillus cocktail exhibits antiproliferative effects against colorectal cancer cells.
  • The mechanism involves the downregulation of the BMP signaling pathway and associated genes, including hes1 and beta-catenin.
  • These findings suggest that this Lactobacillus cocktail holds promise as a preventative agent for colorectal cancer (CRC).