Jove
Visualize
Contact Us

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Protein Networks02:26

Protein Networks

3.9K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hyperactive GNAQ in endothelial cells causes blood vessel malformation in developing mouse skin.

Development (Cambridge, England)·2026
Same author

The Trifecta of Polo-Like Kinases, Cancer, and the Immune System: Emerging Intersections and Therapeutic Insights.

Molecular cancer research : MCR·2026
Same author

Development of Potent and Selective RIPK1 Degraders Targeting Its Nonenzymatic Function for Cancer Treatment.

Journal of medicinal chemistry·2025
Same author

Technical Considerations for Detecting Protein-Protein Interactions Using Proximity Ligation Assay.

Journal of proteome research·2025
Same author

Overactive Wnt5a signaling disrupts hair follicle polarity during mouse skin development.

Development (Cambridge, England)·2022
Same author

Functional redundancy of frizzled 3 and frizzled 6 in planar cell polarity control of mouse hair follicles.

Development (Cambridge, England)·2018
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 13, 2025

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
09:18

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

7.4K

Improved method for detecting protein-protein interactions using proximity ligation assay.

Zach Tower1, Hao Chang1,2

  • 1Department of Dermatology, University of Wisconsin-Madison, Madison, Wisconsin 53705.

Biorxiv : the Preprint Server for Biology
|September 16, 2024
PubMed
Summary

Proximity ligation assays can detect protein-protein interactions but may yield false positives. This study identifies these inaccuracies and offers strategies to improve specificity, particularly for membrane proteins.

Keywords:
Astn2Fzd6Protein-protein interactionplanar cell polarityproximity ligation assay

More Related Videos

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
10:05

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Published on: January 16, 2017

12.8K
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

10.0K

Related Experiment Videos

Last Updated: Jun 13, 2025

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
09:18

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

7.4K
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
10:05

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Published on: January 16, 2017

12.8K
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

10.0K

Area of Science:

  • Molecular biology
  • Cellular biology
  • Biochemistry

Background:

  • Proximity ligation assay (PLA) is a sensitive technique for detecting protein-protein interactions in biological samples.
  • However, the specificity of PLA is often overlooked, leading to potential inaccuracies.
  • False positive signals can complicate the interpretation of protein interaction studies.

Purpose of the Study:

  • To investigate and report the occurrence of false positives in proximity ligation assays.
  • To provide practical recommendations for minimizing false positive signals in PLA.
  • To enhance the accuracy of protein-protein interaction detection, with a focus on membrane proteins.

Main Methods:

  • Utilized proximity ligation assay (PLA) in cellular and tissue contexts.
  • Systematically analyzed and quantified false positive signals.
  • Developed and tested methods to reduce assay-specific false positives.

Main Results:

  • Demonstrated that proximity ligation assays exhibit varying levels of false positive results.
  • Identified specific conditions and factors contributing to these false positives.
  • Validated strategies that effectively reduce false positive rates in PLA.

Conclusions:

  • Proximity ligation assay requires careful optimization to ensure specificity.
  • Implementing suggested modifications can significantly improve the accuracy of protein-protein interaction detection.
  • These findings are crucial for reliable studies involving protein interactions, especially for challenging targets like membrane proteins.