Impact of Acute Dietary and Exercise Manipulation on Next-Day RMR Measurements and DXA Body Composition Estimates

The objective of this study is to investigate the effects of acute diet and exercise manipulation on resting metabolic rate (RMR) measurement variability and dual-energy x-ray absorptiometry (DXA) body composition estimates.

Ten male and 10 female endurance athletes (12 cyclists, 5 triathletes, 4 runners) of tier 2 ( n = 18) to tier 3 ( n = 2) caliber underwent five conditions using a Latin square counterbalance design. For 24 h, athletes consumed a diet providing excessive energy availability (EA) (75 kcal⋅kg fat-free mass (FFM) -1 ) without exercise (GEA rest ), high-EA (45 kcal⋅kg FFM -1 ) without (HEA rest ) or with exercise (HEA ex ), or low-EA (15 kcal⋅kg FFM -1 ) without (LEA rest ) or with exercise (LEA ex ). Exercise involved two bouts of cycling (morning bout: 149 ± 34 min at 55% of maximal aerobic capacity (V̇O 2max ); afternoon bout: 60 min at 65% of V̇O 2max ) that resulted in a cumulative exercise energy expenditure of 30 kcal⋅kg FFM -1 . The following day, RMR and DXA measurements occurred after a 10-h fast and 12-h postexercise.

There were neither sex differences in relative RMR ( P = 0.158) nor effects of any of the five conditions on RMR ( P = 0.358). For both male and female athletes, FFM estimates were decreased following the LEA rest (-0.84 ± 0.66 kg; P = 0.001) and LEA ex (-0.65 ± 0.86 kg; P = 0.016) conditions compared with the GEA rest condition and following the LEA rest (-0.73 ± 0.51 kg; P = 0.001) and LEA ex (-0.54 ± 0.79 kg; P = 0.024) conditions compared with the HEA ex condition. There was no effect of condition on fat mass estimates ( P = 0.819).

Acute periods of diet and exercise manipulation did not create artifacts in next-day RMR measurements. However, as changes in estimates of FFM were seen, diet and exercise should be controlled in the 24-h before DXA scans.