Related Experiment Video

Updated: Jun 13, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
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Completion of the genome sequence of Oidiodendron maius splipalmivirus 1

Stefania Daghino1, Marco Forgia1, Massimo Turina2,3

  • 1Institute for Sustainable Plant Protection, CNR, Strada delle Cacce 73, 10135, Torino, Italy.

Archives of Virology
|September 16, 2024
PubMed

Insights

Researchers identified a new RNA segment for the Oidiodendron maius splipalmivirus 1 (OmSPV1), a mycovirus with a unique split genome structure. This discovery expands our understanding of splittable palm (splipalm) mycovirus genome organization.

Area of Science:

  • Mycology
  • Virology
  • Molecular Biology

Background:

  • Mycoviruses with split RNA-directed RNA polymerase (RdRp) palm domains, termed splipalmiviruses, have been recently discovered in fungi and oomycetes.
  • Oidiodendron maius splipalmivirus 1 (OmSPV1) was previously proposed to have a bisegmented genome.

Purpose of the Study:

  • To complete the genome sequence of OmSPV1.
  • To characterize a newly identified RNA segment of OmSPV1.

Main Methods:

  • Genome sequencing
  • Bioinformatic analysis of RNA segments and open reading frames (ORFs).

Main Results:

  • A third RNA segment of OmSPV1, approximately 2000 nucleotides long, was identified.
  • This segment shares identical terminal sequences with the other two OmSPV1 segments.
  • It contains a single ORF encoding a protein with unknown function, showing 47% sequence identity to a protein from Magnaporthe oryzae narnavirus virus 1 (MoNV1).

Conclusions:

  • The newly identified RNA segment is proposed as the third segment of the OmSPV1 genome.
  • This finding further elucidates the unique genome organization of splipalmiviruses.

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