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Bridging structural biology and clinical research through in-tissue cryo-electron tomography

Kathryn Kixmoeller1,2, Benjamin C Creekmore1,2,3, Edward B Lee3

  • 1Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

The EMBO Journal
|September 16, 2024
PubMed
Abstract

No abstract available in PubMed .

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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