Related Experiment Video
Updated: Jun 13, 2025
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
Published on: December 28, 2017
Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection
Purpose:
Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The "gold standard" culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of C. glabrata.
Methodology:
The specific LAMP primers for C. glabrata detection were designed and evaluated.
Results:
The LAMP assay accurately detected C. glabrata with no cross-reactivity with other Candida species.
Conclusion:
The developed molecular method would be a promising tool in the management of invasive candidiasis.
