Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Asymmetric Lipid Bilayer

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...

Micelles

Micelles

Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Advances in multivariate analysis of extracellular vesicles using visible light-based analytical methods.

Nanoscale·2026

Same author

Microfluidic Generation of Oxidized Phospholipid Gradients in Supported Lipid Bilayers to Examine Scavenger Receptor Interactions.

Analytical chemistry·2025

Same author

Atherosclerotic Oxidized Lipids Affect Formation and Biophysical Properties of Supported Lipid Bilayers and Simulated Membranes.

The journal of physical chemistry. B·2024

Same author

Physicochemical Properties of Seed Oil Blends and Their Potential for the Creation of Synthetic Oleosomes with Modulated Polarities.

ACS omega·2024

Same author

Engineering Planar Gram-Negative Outer Membrane Mimics Using Bacterial Outer Membrane Vesicles.

Langmuir : the ACS journal of surfaces and colloids·2024

Same author

Engineering Planar Gram-Negative Outer Membrane Mimics Using Bacterial Outer Membrane Vesicles.

bioRxiv : the preprint server for biology·2024

Same journal

Laser-Assisted Electrochemical Deposition of Bilateral Au Coatings on Ni Foils: Mechanism and Experimental Study.

Langmuir : the ACS journal of surfaces and colloids·2026

Same journal

Mechanistic Insights into Pulmonary Surfactant Inactivation.

Langmuir : the ACS journal of surfaces and colloids·2026

Same journal

MPN-GE Bilayer Interphase Construction: Green Modification Derived from Biomass and Synergistic Enhancement of CFRP.

Langmuir : the ACS journal of surfaces and colloids·2026

Same journal

Magnetically Retrievable Core@Shell Nanocomposites for Rare Earth Element Adsorption: Experimental and Machine Learning Insights.

Langmuir : the ACS journal of surfaces and colloids·2026

Same journal

Microstreaming of a Pneumatically Controlled Bubble under Hydrostatic Pressure and Crossflow.

Langmuir : the ACS journal of surfaces and colloids·2026

Same journal

Tuning Pore Sizes of Core-Shell Dendritic Mesoporous Silica Nanoparticles for Efficient Loading of Functional Materials.

Langmuir : the ACS journal of surfaces and colloids·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 9, 2026

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

Published on: December 1, 2015

Chaotropic Agent-Assisted Supported Lipid Bilayer Formation.

Jennie L Cawley¹, Dane E Santa¹, Aarshi N Singh¹

¹Department of Chemistry, Lehigh University, 6 East Packer Avenue, Bethlehem, Pennsylvania 18015, United States.

Langmuir : the ACS Journal of Surfaces and Colloids

|September 17, 2024

Summary

This summary is machine-generated.

A new all-aqueous chaotropic agent exchange method creates supported lipid bilayers (SLBs) on diverse surfaces. This technique offers a versatile alternative for forming these biomimetic membranes, overcoming limitations of traditional vesicle fusion methods.

More Related Videos

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

Published on: August 25, 2023

Related Experiment Videos

Last Updated: Jun 9, 2026

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

Published on: December 1, 2015

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

Published on: August 25, 2023

Area of Science:

Biomaterials Science
Surface Chemistry
Membrane Biophysics

Background:

Supported lipid bilayers (SLBs) are crucial for mimicking cellular membranes and integrating biosensors.
Existing SLB formation methods face limitations regarding lipid composition and substrate compatibility.

Purpose of the Study:

To introduce an all-aqueous chaotropic agent exchange process for SLB formation.
To demonstrate the versatility of this method on various substrates, including those incompatible with traditional techniques.

Main Methods:

Utilized an all-aqueous chaotropic agent exchange process.
Formed SLBs on SiO2 and Al2O3 substrates.
Characterized SLBs using quartz crystal microbalance with dissipation monitoring and fluorescence microscopy.

Main Results:

Chaotropic agent exchange-supported lipid bilayers (CASLBs) exhibited similar properties to those formed by vesicle fusion.
CASLBs effectively prevented nonspecific protein adsorption.
Long-range lateral diffusion confirmed the continuous, planar nature of CASLBs.

Conclusions:

The chaotropic agent exchange method provides a new, adaptable approach for creating supported lipid bilayers.
This technique expands the utility of SLBs to a broader range of surfaces, including those not suitable for vesicle fusion.