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Nitric oxide-dependent proton translocation in various denitrifiers.

J P Shapleigh, W J Payne

    Journal of Bacteriology
    |September 1, 1985
    PubMed
    Summary

    Nitric oxide (NO) respiration drives proton translocation in diverse denitrifiers. This process suggests a unique NO reductase enzyme present in these anaerobic bacteria.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Environmental Science

    Background:

    • Denitrification is a crucial microbial process in nitrogen cycling.
    • Nitric oxide (NO) is an intermediate in denitrification, and its role in proton translocation is not fully understood.
    • Understanding NO metabolism is vital for comprehending microbial respiration and environmental nitrogen transformations.

    Purpose of the Study:

    • To investigate the proton translocation associated with nitric oxide (NO) respiration in various denitrifying bacteria.
    • To determine the stoichiometry of proton movement per molecule of NO reduced.
    • To explore the potential presence and function of a novel NO reductase.

    Main Methods:

    • Respiration experiments using anaerobically grown cultures of four diverse denitrifying species.
    • Measurement of proton translocation (H+/NO ratios) during NO exposure.
    • Inhibition studies using Antimycin A to elucidate proton consumption pathways.
    • Analysis of proton uptake with artificial electron donors in inhibited cells.

    Main Results:

    • Transient proton translocation was observed in all tested denitrifiers upon NO respiration.
    • H+/NO ratios varied among species, ranging from 1.12 to 4.96.
    • Antimycin A significantly inhibited NO-dependent proton translocation, particularly in *Paracoccus denitrificans*.
    • Evidence for periplasmic proton consumption during NO reduction was obtained.

    Conclusions:

    • The data support the existence of a unique NO reductase in anaerobically grown denitrifying bacteria.
    • Proton translocation is directly linked to NO reduction to nitrous oxide (N2O) or nitrogen gas (N2).
    • This finding contributes to understanding the bioenergetics of denitrification and microbial adaptation.

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