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OSR1 suppresses oral squamous cell carcinoma proliferation and migration via the AXIN2/β-catenin pathway

Xintong Guo1, Xinyi Du1, Gaoye Zhao1

  • 1School of Stomatology, North China University of Science and Technology, Tangshan, Hebei, China.

Oral Diseases
|September 17, 2024
PubMed
Abstract

Insights

Odd-skipped related transcription factor 1 (OSR1) acts as a tumor suppressor in oral squamous cell carcinoma (OSCC). Lower OSR1 levels correlate with poor prognosis, while its overexpression inhibits OSCC cell proliferation and migration.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • The odd-skipped related transcription factor 1 (OSR1) gene influences tumorigenesis and development across various cancers.
  • Its specific role in oral squamous cell carcinoma (OSCC) requires further investigation.

Purpose of the Study:

  • To elucidate the function of OSR1 in oral squamous cell carcinoma (OSCC).
  • To analyze the impact of OSR1 expression on patient prognosis and cellular behavior in OSCC.

Main Methods:

  • Analysis of OSR1 expression and prognostic correlation in head and neck squamous cell carcinoma (HNSC) using GEPIA 2 and TCGA databases.
  • Detection of OSR1 in OSCC tissues and cells via immunohistochemistry, immunofluorescence, western blotting, and RT-qPCR.
  • Functional assays including cell proliferation, colony formation, and migration assays following OSR1 manipulation (overexpression/downexpression) using lentivirus transfection.

Main Results:

  • OSR1 expression was significantly reduced in HNSC patients and OSCC tissues/cells, correlating with a lower 5-year survival rate.
  • Overexpression of OSR1 suppressed OSCC cell proliferation and migration.
  • OSR1 manipulation affected the AXIN2/β-catenin signaling pathway, with inhibition observed upon OSR1 overexpression.

Conclusions:

  • OSR1 functions as a tumor suppressor gene in OSCC.
  • OSR1 inhibits OSCC cell proliferation and migration by regulating the AXIN2/β-catenin signaling pathway.
Keywords:
AXIN2/β‐catenin pathwayOSCCOSR1

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