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Targeting TRIM26: Unveiling an Oncogene and Identification of Plant Metabolites as a Potential Therapeutics for

Sweta H Makwana1, Tannavi Sharma1, Manas K Mahapatra1

  • 1Department of Biochemistry, School of Life Sciences, Central University of Rajasthan, Ajmer, Rajasthan, India.

Journal of Cellular Biochemistry
|September 17, 2024
PubMed

Insights

Tripartite Motif 26 (TRIM26) is upregulated in breast cancer and drives cancer cell proliferation and metastasis. Silencing TRIM26 inhibited these processes, suggesting it as a potential therapeutic target for breast cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer is a leading cause of cancer mortality globally.
  • The Tripartite Motif (TRIM) protein family regulates cancer growth and metastasis but remains underexplored in cancer cell regulation.
  • TRIM26, a member of the TRIM family, is upregulated in numerous cancer types, including breast cancer.

Purpose of the Study:

  • To investigate the role of TRIM26 in breast cancer progression.
  • To determine if targeting TRIM26 could offer a novel therapeutic strategy.

Main Methods:

  • Analysis of TRIM gene expression in a breast cancer database.
  • siRNA-mediated gene silencing of TRIM26 in MCF-7 and MDA-MB 231 breast cancer cell lines.
  • Assessment of cell proliferation, migration, invasion, and expression of key markers.
  • In silico molecular docking studies.

Main Results:

  • 16 out of 77 TRIM genes were found to be upregulated in breast cancer.
  • TRIM26 expression was significantly reduced following siRNA silencing.
  • Reduced TRIM26 expression led to decreased cancer cell proliferation, migration, and invasion.
  • Silencing TRIM26 modulated proliferative, cell cycle, mesenchymal, and epithelial markers.
  • Docking studies identified potential plant-derived therapeutic compounds.

Conclusions:

  • TRIM26 plays a crucial role in promoting breast cancer cell proliferation, migration, and invasion.
  • Targeting TRIM26 presents a promising therapeutic avenue for breast cancer treatment.
  • Novel plant metabolites show potential for inhibiting TRIM26 activity.
Keywords:
TCGA analysisTRIM26breast cancerepithelial to mesenchymal transitionmolecular docking

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