Related Experiment Video
Updated: Jun 13, 2025

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Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
Published on: February 4, 2018
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Broad range molecular detection methods identify only Borrelia spp. in erythema migrans biopsies and blood of
Philippe Pérot1,2, Laura Tondeur3, Sara Moutailler4
1Pathogen Discovery Laboratory, Institut Pasteur, Université Paris Cité, F-75015 Paris, France.
One Health (Amsterdam, Netherlands)
|September 17, 2024
Abstract:
In this multicenter study conducted in France, we challenged the hypothesis of the transmission of pathogens other than Borrelia spp. in 22 patients developing erythema migrans following a tick bite. Using a combination of high-throughput microfluidic PCRs and agnostic metagenomics on skin biopsies and blood samples, no microorganisms other than Borrelia spp. was found.

