Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ratiometric pH-Responsive and Biomimetic Microparticles for Quantitative Monitoring of Phagosomal Acidification.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

A Microfluidic Platform for Actin-Based Membrane Remodeling Reveals the Stabilizing Role of Branched Actin Networks on Lipid Microdomains.

Small science·2025
Same author

Pneumococcal competence is a populational health sensor driving multilevel heterogeneity in response to antibiotics.

Nature communications·2024
Same author

Observation of Ultra-High-Q Resonators in the Ultrasound via Bound States in the Continuum.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2024
Same author

IpaA reveals distinct modes of vinculin activation during <i>Shigella</i> invasion and cell-matrix adhesion.

Life science alliance·2024
Same author

FRET-Sensing of Multivalent Protein Binding at the Interface of Biomimetic Microparticles Functionalized with Fluorescent Glycolipids.

ACS applied materials & interfaces·2024

Related Experiment Video

Updated: Jul 3, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K

Functionalization of microbubbles in a microfluidic chip for biosensing application.

Marc Prudhomme1, Chaimaa Lakhdar1, Jacques Fattaccioli2,3

  • 1Institut FEMTO-ST, Université de Franche-Comté, CNRS, Besançon, F-25000, France.

Biomedical Microdevices
|September 17, 2024
PubMed
Summary

This study presents a rapid microbubble functionalization protocol for biosensors. The new method, utilizing microfluidics, enables quick capture and regeneration cycles under 10 minutes.

Keywords:
BiosensorMicrobubbleReusableSurface functionalization

More Related Videos

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.1K
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)&#8211;Cell Interaction and the Resultant Bioeffects at the Single-cell Level
11:14

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

11.7K

Related Experiment Videos

Last Updated: Jul 3, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.1K
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)&#8211;Cell Interaction and the Resultant Bioeffects at the Single-cell Level
11:14

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

11.7K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Biochemistry

Background:

  • Microbubbles are versatile tools in biomedical imaging and therapy.
  • Functionalizing microbubbles for targeted applications is challenging due to interface instability.
  • Existing biosensors often lack rapid regeneration capabilities.

Purpose of the Study:

  • To develop a simple and rapid protocol for microbubble functionalization.
  • To integrate functionalized microbubbles into a novel microfluidic biosensor.
  • To demonstrate the biosensor's capability for rapid analyte detection and surface regeneration.

Main Methods:

  • Microbubbles functionalized with DSPE-PEG-Biotin via direct generation in a microfluidic chip.
  • Organizing functionalized microbubbles in a chamber for bioanalyte capture.
  • Utilizing fluorescent microscopy to assess functionalization and streptavidin capture.
  • Demonstrating a complete functionalization-capture-measurement cycle under 10 minutes.

Main Results:

  • Successful rapid functionalization of microbubbles directly during generation.
  • Demonstrated capture of streptavidin as a model bioanalyte using functionalized microbubbles.
  • Achieved a complete biosensing cycle (functionalization, capture, measurement, regeneration) in under 10 minutes.
  • Verified functionalization and capture using fluorescent microscopy.

Conclusions:

  • The developed microfluidic protocol enables efficient and rapid microbubble functionalization.
  • The novel microbubble-based biosensor offers significant advantages in speed and regeneration.
  • This technique holds promise for developing next-generation diagnostic and therapeutic tools.