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CD24-Targeted NIR-II Fluorescence Imaging Enables Early Detection of Colorectal Neoplasia
Xiaoyong Guo1,2, Shuangling Luo3, Xiaofeng Wang4
1Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Gastrointestinal Cancer Center, Ward I, Peking University Cancer Hospital & Institute, Beijing, China.
Cancer Research
|September 17, 2024
Summary
A new fluorescent probe targets CD24, a protein overexpressed in colorectal cancer. This imaging method enhances early detection of colorectal neoplasia, including small lesions missed by standard colonoscopy.
Area of Science:
- Biomedical imaging
- Molecular imaging
- Gastroenterology
Background:
- Colorectal cancer remains a significant health concern despite advances in early detection.
- White-light colonoscopy often misses subtle, invasive lesions, leading to advanced tumor development.
- Fluorescence molecular imaging offers improved detection by targeting specific molecular markers.
Purpose of the Study:
- To develop and evaluate a novel second near-infrared window (NIR-II) fluorescent probe targeting CD24 for colorectal cancer detection.
- To assess the probe's efficacy in identifying colorectal neoplasia, including early-stage and small lesions.
Main Methods:
- Development of a CD24-targeting NIR-II fluorescent probe.
- Evaluation in cellular models, murine models, and human colorectal cancer samples.
- Comparison with conventional near-infrared window (NIR-I) imaging and white-light detection.
Main Results:
- CD24 was overexpressed in 76% of adenomas and 80% of colorectal cancers.
- NIR-II imaging with the CD24 probe showed a higher tumor-to-background ratio than NIR-I imaging.
- The probe achieved 92% sensitivity and 92% specificity, detecting lesions <1 mm and those missed by other methods.
Conclusions:
- CD24-targeted NIR-II imaging is effective for detecting colorectal neoplasia, including occult lesions.
- This approach holds significant potential for improving early colorectal cancer diagnosis.
- Topical probe application may enable intraprocedural lesion identification during colonoscopy.
